《植物生理学报》 2017, 53(6): 1097-1103

施氮对不同分隔方式间作蚕豆根系分泌柚皮素的影响

刘英超, 肖靖秀, 汤利, 郑毅^*

云南农业大学资源与环境学院, 昆明650201

通信作者：郑；E-mail: yzheng@ynau.edu.cn

摘　要：

合理间作可以促进豆科植物结瘤, 促进与之间作作物氮素的固定和利用。柚皮素作为能被根瘤菌识别的重要根系分泌物质, 在豆科作物根瘤的形成过程中具有十分重要的作用, 但施氮量和根系分隔方式对间作蚕豆根系分泌柚皮素的变化尚不清楚。本文通过盆栽和水培试验, 采用尼龙分隔(MB)、塑料分隔(PB)、无分隔(NB)三种分隔方式, 研究了小麦蚕豆间作在不同氮水平条件下, 蚕豆根系柚皮素分泌量及分泌速率。结果表明, 低氮条件下, 与塑料分隔(PB)相比, 尼龙分隔(MB)柚皮素分泌量提高了34.1%, 分泌速率提高了31.1%；无分隔(NB)柚皮素分泌量提高了54.6%, 分泌速率无明显差异。推荐施氮条件下, 尼龙分隔(MB)和无分隔(NB)柚皮素的分泌量提高了67%和24.6%, 不同根系分隔方式对蚕豆柚皮素的分泌速率没有明显影响。高氮条件下, 三种分隔方式间差异不显著, 但分泌速率较低氮和推荐施氮条件明显减小。总之, 施氮和间作根系分隔方式能影响蚕豆植物根系柚皮素的分泌。

关键词：氮; 分隔方式; 蚕豆; 柚皮素

收稿：2017-02-13   修定：2017-05-02

资助：国家自然科学基金(31460551、41361065和31260504)。

Effects of nitrogen application rate on the naringenin exudation from intercropped faba bean’s roots in different separation patterns

LIU Ying-Chao, XIAO Jing-Xiu, TANG Li, ZHENG Yi^*

College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China

Corresponding author: ZHENG Yi; E-mail: yzheng@ynau.edu.cn

Abstract:

Rational intercropping can promote nodulation of legumes and facilitate the fixation and utilization of nitrogen between crops. As an important root secreted material that identified by rhizobium, naringenin plays a very important role in the formation of root nodules of legumes, but how to nitrogen levels affect naringenin, it is not clear. By pot and water cultural experiments of wheat//faba bean, used mesh barrier (MB), polythene barrier (PB), and no barrier (NB) separation patterns, secretion and secretion rate of naringenin of faba bean were studied at different nitrogen levels in wheat and faba bean intercropping system. The results showed that, under the condition of nitrogen deficiency, compared with PB, secretion of naringenin in MB system was increased by 34.1%, and secretion rate increased by 31.1%; in NB system, secretion of naringenin was increased by 54.6%, but there was no significant change in secretion rate. Under the condition of nitrogen adequate, secretion of naringenin in MB and NB system were increased by 67% and 24.6%, while secretion rate were not obviously affected in different separation systems. Under the condition of high nitrogen, the differences among three separation patterns are not significant, but the secretion rates were decreased significantly compared to deficiency and adequate nitrogen obviously. In short, nitrogen fertilizer and roots separation in intercropping system could change the secretion of naringenin secreted by faba bean.

Key words: nitrogen; root barriers; faba bean; naringenin